Profiling of cellular signal transduction pathways using a bead-based Western-blot*
نویسندگان
چکیده
منابع مشابه
A bead-based western for high-throughput cellular signal transduction analyses
Dissecting cellular signalling requires the analysis of large number of proteins. The DigiWest approach we describe here transfers the western blot to a bead-based microarray platform. By combining gel-based protein separation with immobilization on microspheres, hundreds of replicas of the initial blot are created, thus enabling the comprehensive analysis of limited material, such as cells col...
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ژورنال
عنوان ژورنال: EuPA Open Proteomics
سال: 2014
ISSN: 2212-9685
DOI: 10.1016/j.euprot.2013.11.007